restriction enzyme analysis meaning in English
限制酶分析
Examples
- The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod
结果表明同源性分别达到98和97 ，并且在ha1切割位点有多个碱性氨基酸的插入序列，证明其为强毒株。 - For the products of primary rt - pcr and nested - pcr are all encompassing the hyper - variable region of vp2 gene , so we can take a restriction enzyme analysis ( rea ) directly
第二，本研究的基础rt - pcr和nested - pcr所扩增的片段均是横跨ibdv的vvp2的，所以，可直接对pcr产物进行酶切分型研究。 - Proper multi - copy gene was selected and cloned into puc19 vector . restriction enzyme analysis and dna sequencing confirmed that 5 - copy gene was correctly inserted into the vector
选取合适拷贝数的串连重复基因，将其克隆至puc19载体，双酶切、 pcr扩增和dna测序证明串连重复基因构建成功且基因方向相同。 - We confirmed the correct construction by pcr and restriction enzyme analysis . in this research , hypocotyls were used as the explant and several factors affecting genetic transformation of carrot mediated by agrobacterium tumefaciens were studied
利用vp7基因和质粒pbi121上相同的单克隆位点，将vp7基因定向克隆到植物表达载体pbi121上，构建了pbi121vp7表达载体。 - Conclusion : by restriction enzyme secting , ligating , transforming , restriction enzyme analysis , and final dna sequencing , the pbd - i and pbd - ii gene were proved to be recombinated with the expression vector and the recombinated vector ppd - 1 and ppd2 were transformed successfully
结论：经过酶切、连结，构建成重组质粒ppd上、 ppd上，再经转化、抽提质粒及酶切分析，最后经dna测序证实， rticr扩增的pbd i 、 pbd 11基因与piflpdt ” xsi表达载体构建成功。